Е.В. Фатеева, Е.В. Мокшин, А.С. Лукаткин.
Влияние концентрации минеральной основы среды и регуляторов роста на органогенез цимбидиума гибридного в культуре in vitro
// Труды КарНЦ РАН. No 3. Сер. Экспериментальная биология. 2013. C. 143-148
E.V. Fateeva, E.V. Mokshin, A.S. Lukatkin. Effects of the concentrations of the medium inorganic base and growth regulators on cymbidium hybrid organogenezis in vitro // Transactions of Karelian Research Centre of Russian Academy of Science. No 3. Experimental biology. 2013. Pp. 143-148
Keywords: Cymbidium hybrids; in vitro; Murashige-Skoog media; inorganic base; kinetin; thidiazuron; benzyladenine; IAA; organogenesis; pseudobulbs; growth
The effects of the concentration of the inorganic base of Murashige-Skoog (MS) media and synthetic plant growth regulators (PGR) – cytokinins (kinetin, 6-BAP or thidiazuron) with IAA – on Cymbidium hybrid organogenesis in vitro are presented. In the experiment with varying concentrations of the MS mineral base we revealed that the growth of shoots was maximal on the complete MS medium, whereas rooting and pseudobulbs formation – on 1/2 MS. In the combined PGR and auxin treatments the best effect on the formation of pseudobulbs in the Cymbidium hybrid was achieved in media supplemented with 2.5 mg/l kinetin + 0.5 mg/l IAA (pseudobulbs number) and 0.1 mg/l kinetin + 0.5 mg/l IAA (pseudobulbs size). The number of newly formed shoots was maximal on the medium supplemented with 10-4 mol/l thidiazuron + 0.5 mg/l IAA, whereas the longest shoots formed on media supplemented with 6BAP + IAA (0.5 mg/l each).
