Data on two vascular plant species new to Russian flora and other 32 notable regional records made in seven regions of European Russia (Vologda Region, Kostroma Region, Leningrad Region, Pskov Region, Yaroslavl Region, Saint-Petersburg and Udmurt Republic) are given. The presented material is derived from the floristic inventories conducted in 2006-2017, and critical study of the available herbarium. Euphrasia micrantha Rchb. and Senecio inaequidens DC. are reported as new to Russia, the first species as native and the secong one as alien. Bupleurum falcatum L. is reported as a new alien species for the flora of NW European Russia. Bidens frondosa L. is new for the Pskov Region. Cardamine flexuosa With. is reported for the Yaroslavl Region for the first time. Eight taxa are reported as new to the Kostroma Region, five of them (Alnus x hybrida R. Br. ex Rchb., Carex bohemica Schreb., Cladium mariscus (L.) Pohl, Sedum maximum (L.) Hoffm. and Urtica galeopsifolia Wierzb. ex Opiz) considered native, and three others (Collomia linearis Nutt., Gypsophila elegans Bieb. and Silene armeria L.) as alien. Dryopteris x uliginosa (A. Br. ex Doll) Kuntze ex Druce is a new hybrid for the Kostroma Region, Leningrad Region and Udmurt Republic. In addition, 20 species with lacking regional records are reported to. The data presented points to a continuing spreading of alien plants across European Russia. On the other hand, the native flora of some regions is still poorly investigated.