Information is reported about 85 species of macrofungi found in the City of Petrozavodsk for the first time during surveys carried out in 2009–2018. The list includes 45 mycorrhizal species (apart from 2 facultative species – Clitocybe nebularis and Inocybe acuta) and 40 saprotrophic species (10 humus-dwelling, 9 forest floor dwellers, 18 xylotrophs, 2 species dwelling on macrofungal fruit bodies, and 1 on litterfall). There were 2 species registered for Karelia for the first time – Leccinum albostipitatum and Lycoperdon echinatum. Two species are listed in the Red Data Book of the Republic of Karelia – Amanita virosa and Imleria badia (category: rare, potentially vulnerable). With the new findings, the biota of macrofungi in Petrozavodsk comprises 196 species, i. e. 28.5 % of the total number of species of this fungal group known from the Republic of Karelia. Information about the trophic affiliations, habitats and distribution is given for each taxon.