An annotated list of bryophytes of the Buzuluk Bor National Park within Orenburg Region was compiled based on field observations, herbarium collections, and data from the literature. This territory comprises the largest pine forest in the steppe zone of Northern Eurasia and the only “island” forest area with relict landscapes in steppes on the left bank of the Volga River. The bryophyte flora of this territory is made up of
18 liverworts and 68 mosses; six of the moss species are regionally red-listed. Two previous records (Grimmia plagiopodia Hedw., Polytrichum strictum Brid.) are questionable.