An updated checklist of chewing lice (Phthiraptera: Mallophaga, Amblycera) of the Voronezh Region was compiled based on an inventory of the entomological material collected in 1976-2021. It contains 30 species of chewing lice of the suborder Amblycera. They belong to two families and 12 genera. Among them, 16 species, – Meromenopon incisum (Giebel, 1874), Trinoton querquedulae (Linnaeus, 1758), Austromenopon icterum (Burmeister, 1838), Menacanthus alaudae (Schrank, 1776), M. pici (Denny, 1842), Colpocephalum fregili Denny, 1842, C. turbinatum Plaget, 1880, Myrsidea cucullaris (Nitzsch, 1818), M. isostoma (Nitzsch, 1866), M. indivisa (Nitzsch, 1866), M. troglodyti (Denny, 1842), Bonomiella columbae Emerson, 1957, B. concii Eichler, 1947, Hohorstiella lata (Piaget, 1880), H. modesta (Ansari, 1951), Ricinus serratus Durrant, 1906 – are registered in the Voronezh Region for the first time. Genera Menacanthus and Myrsidea comprise the highest number of species. Most chewing lice species were associated with their typical host-species. However, in urban ecosystems of the region, several Amblycera species (Menacanthus agilis, M. gonophaeus, Colpocephalum turbinatum) were retrieved from occasional host species.