A comparative analysis of gene expression encoding proteins involved in the regulation of immune response and inflammatory reactions in the group of patients with pulmonary sarcoidosis (PS) with chronic course of the disease (stage II), untreated and healthy people (control) was carried out. Changes in the expression of TLR2, TLR4, FOXP3, RORC, NLRP3 genes in peripheral blood leukocytes (PBL) of lung sarcoidosis patients with chronic disease progression, without therapy compared to healthy people were found. The level of transcripts of Toll-like receptor genes (TLR2 and TLR4) in PBL of patients with pulmonary sarcoidosis was higher than in healthy people (p < 0.001). An increase in the mRNA content of FOXP3, RORC, NLRP3 genes in the Pbmcs of patients compared to healthy individuals was revealed (p < 0.01). In the group of patients with pulmonary sarcoidosis a close correlation between the level of RORC gene expression and the content of gene FOXP3 (r = 0.74; p = 0.008), TLR4 (r = 0.74; p = 0.008), TLR2 (r = 0.76; p = 0.006) transcripts was revealed. In the same group of examined individuals, a positive correlation was found between the expression level of TLR4 and FOXP3 genes (r = 0.578; p = 0.037), TLR2 and TLR4 genes (r = 0.64; p = 0.02). The increased content of FOXP3, RORC gene transcripts in the PBL of PS patients with stable course without therapy in comparison with healthy individuals and their positive correlation with the expression level of TLR2 and TLR4 genes indicate the activation of adaptive and innate immunity cells and the development of chronic inflammation in this disease.