The study evaluated the effect of supplementing fish feed with a complex of plant-based additives combined with antimicrobial peptide Pediocin PA-1. The object of the study is the hybrid sturgeon species LenKa, obtained by crossing the Lena sturgeon and Kaluga (Acipenserbaerii × Husodauricus) to enhance their characteristics, such as growth, disease resistance and egg quality. The methods included producing a pilot sample of feed with the new additive and laboratory studies of the nutritional value and amino acid composition of the experimental batch for compliance with requirements for compound feeds for sturgeon hybrids. The new feed formula supplemented with antimicrobial peptide Pediocin PA-1 has a positive effect on the general wellbeing of the fish and provides a weight gain. The proposed feed is characterized by high palatability for aquatic organisms, as confirmed by the absence of visually detectable feed residues in the aquatic environment. This helps to minimize the risk of water pollution by feed decay products and thus to maintain stable hydrochemical parameters. In many countries, aquaculture plays a key role in providing local populations with high-quality fish produce. Over the past decades, production volumes in this industry have increased enormously, approximately 12-fold. A crucial factor for obtaining high-quality and cost-effective fish products is a balanced diet of feeds containing all the necessary nutritional and biologically active elements. The new supplement will expand the range of aquaculture feeds in the market and reduce water treatment costs by minimizing waste.