И.В. Суховская, Е.В. Борвинская, Л.П. Смирнов, Н.Н. Немова.
Сравнительный анализ методов определения концентрации белка – спектрофотометрии в диапазоне 200–220 нм и по бредфорд
// Труды КарНЦ РАН. No 2. Сер. Экспериментальная биология. 2010. C. 68-71
I.V. Sukhovskaya, E.V. Borvinskaya, L.P. Smirnov, N.N. Nemova. Comparative analysis of the methods for determination of protein concentration – spectrophotometry in the 200–220 nm range and the bradford protein assay // Transactions of Karelian Research Centre of Russian Academy of Science. No 2. Experimental biology. 2010. Pp. 68-71
Keywords: proteins, peptides, spectrophotometry, extinction coefficient, Bradford protein assay, bovine serum albumin, glutathione
Results of the comparative analysis of two methods for determination of protein concentration in a solution – direct spectrophotometry at a wavelength of 200–220 mn, and the Bradford protein assay – are reported. It is shown that the extinction coefficient at 205 nm is the most suitable for total protein and peptide determination irrespective of their amino acid composition. To estimate the concentration of low-molecular peptide compounds we suggest using their optical spectrophotometry in the 210–220 nm region.
