И.Л. Голованова, А.А. Филиппов.
Характеристика гликозидаз кишечника лещей Abramis brama (L.) из участков рыбинского водохранилища с различной антропогенной нагрузкой
// Труды КарНЦ РАН. No 2. Сер. Экспериментальная биология. 2012. C. 63-69
I.L. Golovanova, A.A. Filippov. Characteristics of intestinal glycosidases in bream from the Rybinskoye reservoir sites differing in anthropogenic loading // Transactions of Karelian Research Centre of Russian Academy of Science. No 2. Experimental biology. 2012. Pp. 63-69
Keywords: glycosidase, digestion, fish, anthropogenic pollution
The activity, temperature and kinetic characteristics of the intestinal mucosa and cheme glycosidases were investigated in vitro in bream Abramis brama (L.) from parts of the Rybinskoye storage reservoir differing in anthropogenic loading. Considerable similarity was found in the enzymatic activity level and the temperature optimum of glycosidases in bream from the polluted Sheksna and the relatively clean Mologa pools. The lower maltase activity at a temperature of 0–20 С and the higher values of glycosidases Eact in bream from Sheksninsky pool testify to a decrease in the efficiency of food carbohydrate hydrolysis, but the increase in the enzyme=substratum affinity suggests this parameter changes as an adaptation to growing human pressure.